Systems-wide analysis of human stem cell differentiation published in Science Signaling

The Department of Proteomics (Jesper V. Olsen and Matthias Mann) has in collaboration with the group of Associate Prof. Dr. Blagoy Blagoev at the Center for Experimental BioInformatics at the University of Southern Denmark and other international partners published a paper in Science Signaling and made the cover page:

Dynamics of the Stem Cell Phosphoproteome

Science Signaling, 15 March 2011:

Understanding the signaling events that control stem cell pluripotency and self-renewal and those governing differentiation should improve our ability to develop stem cell-based therapies. Rigbolt et al. performed global quantitative proteomic and phosphoproteomic analysis of human embryonic stem cells at five time points over 24 hours of nondirected (lineage-independent) differentiation initiated by two different paradigms. They identified a common core phosphoproteome associated with both differentiation protocols, discovered several temporal patterns of phosphorylation, and made predictions about changes in the activities of kinases during the differentiation period. DNA methyltransferases (DNMTs) exhibited dynamic changes in phosphorylation status that may influence their interaction with a promoter-bound protein complex, suggesting that the phosphorylation state of DNMTs may govern their recruitment to and thus silencing of target genes, such as those that promote pluripotency, during differentiation.

Citation: K. T. G. Rigbolt, T. A. Prokhorova, V. Akimov, J. Henningsen, P. T. Johansen, I. Kratchmarova, M. Kassem, M. Mann, J. V. Olsen, B. Blagoev, System-Wide Temporal Characterization of the Proteome and Phosphoproteome of Human Embryonic Stem Cell Differentiation. Sci. Signal. 4, rs3 (2011).
E-mail: bab@bmb.sdu.dk (B.B.); jesper.olsen@cpr.ku.dk (J.V.O.)