The Danish Cryo-EM Facility - Copenhagen node

The Danish Cryo-EM Facility offers access to state-of-the-art equipment to collect data in single particle  and tomography. The Facility has two nodes, one at University at Copenhagen and one at Aarhus University. The Copenhagen node is placed at the Core Facility for Integrated Microscopy (CFIM) at the Faculty of Health and Medial Sciences, and coordinated by CPR and Guillermo Montoya.

The Titan Krios G2 is a powerful and flexible high resolution electron microscope for 3D characterization of biological samples. It was funded by a grant from The Novo Nordisk Foundation and was inaugurated in 2017. You can read more about the instrument at University of Copenhagen here.

The facility is open for all users, access is granted based on applications submitted through the Cryo-EM Application Portal Denmark.

Scientific output:

 

Conformational landscape of the type V-K CRISPR-associated transposon integration assembly. Tenjo-Castaño F, Sofos N, Stutzke LS, Temperini P, Fuglsang A, Pape T, Mesa P, Montoya G. Mol Cell. 2024 May 29:S1097-2765(24)00398-8. doi: 10.1016/j.molcel.2024.05.005. Online ahead of print. PMID: 38834066

Retron-Eco1 assembles NAD+-hydrolyzing filaments that provide immunity against bacteriophages. Carabias A, Camara-Wilpert S, Mestre MR, Lopéz-Méndez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G. Mol Cell. 2024 Jun 6;84(11):2185-2202.e12. doi: 10.1016/j.molcel.2024.05.001. Epub 2024 May 23. PMID: 38788717ç

Structural insights into the role of GTPBP10 in the RNA maturation of the mitoribosome. Nguyen TG, Ritter C, Kummer E. Nat Commun. 2023 Dec 2;14(1):7991. doi: 10.1038/s41467-023-43599-z.

Diverse roles of the metal binding domains and transport mechanism of copper transporting P-type ATPases. Guo Z, Orädd F, Bågenholm V, Grønberg C, Ma JF, Ott P, Wang Y, Andersson M, Pedersen PA, Wang K, Gourdon P. Nat Commun. 2024 Mar 27;15(1):2690. doi: 10.1038/s41467-024-47001-4.

Structural and mechanistic basis of substrate transport by the multidrug transporter MRP4. Bloch M, Raj I, Pape T, Taylor NMI. Structure. 2023 Nov 2;31(11):1407-1418.e6. doi: 10.1016/j.str.2023.08.014. Epub 2023 Sep 7.

Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit. Hu H, Popp PF, Santiveri M, Roa-Eguiara A, Yan Y, Martin FJO, Liu Z, Wadhwa N, Wang Y, Erhardt M, Taylor NMI.Nat Commun. 2023 Jul 27;14(1):4411. doi: 10.1038/s41467-023-39899-z.

Cryo-EM structure of the trehalose monomycolate transporter, MmpL3, reconstituted into peptidiscs. Couston J, Guo Z, Wang K, Gourdon P, Blaise M. Curr Res Struct Biol. 2023 Nov 8;6:100109. doi: 10.1016/j.crstbi.2023.100109. eCollection 2023.

Endothelial protein C receptor binding induces conformational changes to severe malaria-associated group A PfEMP1. Rajan Raghavan SS, Turner L, Jensen RW, Johansen NT, Jensen DS, Gourdon P, Zhang J, Wang Y, Theander TG, Wang K, Lavstsen T. Structure. 2023 Oct 5;31(10):1174-1183.e4. doi: 10.1016/j.str.2023.07.011. 

Cannabinoid non-cannabidiol site modulation of TRPV2 structure and function. Zhang L, Simonsen C, Zimova L, Wang K, Moparthi L, Gaudet R, Ekoff M, Nilsson G, Hellmich UA, Vlachova V, Gourdon P, Zygmunt PM. Nat Commun. 2022 Dec 5;13(1):7483. doi: 10.1038/s41467-022-35163-y.

Cryo-EM reveals the conformational epitope of human monoclonal antibody PAM1.4 broadly reacting with polymorphic malarial protein VAR2CSA. Raghavan SSR, Dagil R, Lopez-Perez M, Conrad J, Bassi MR, Quintana MDP, Choudhary S, Gustavsson T, Wang Y, Gourdon P, Ofori MF, Christensen SB, Minja DTR, Schmiegelow C, Nielsen MA, Barfod L, Hviid L, Salanti A, Lavstsen T, Wang K. PLoS Pathog. 2022 Nov 16;18(11):e1010924. doi: 10.1371/journal.ppat.1010924. 

TnpB structure reveals minimal functional core of Cas12 nuclease family. Sasnauskas G, Tamulaitiene G, Druteika G, Carabias A, Silanskas A, Kazlauskas C, Venclovas Č, Montoya G, Karvelis T, Siksnys V.  Nature (2023) doi: 10.1038/s41586-023-05826-x.

Cryo-EM reveals the conformational epitope of human monoclonal antibody PAM1.4 broadly reacting with polymorphic malarial protein VAR2CSA. Raghavan SSR, Dagil R, Lopez-Perez M, Conrad J, Bassi MR, Quintana MDP, Choudhary S, Gustavsson T, Wang Y, Gourdon P, Ofori MF, Christensen SB, Minja DTR, Schmiegelow C, Nielsen MA, Barfod L, Hviid L, Salanti A, Lavstsen T, Wang K. PLoS Pathog. 2022 Nov 16;18(11):e1010924. doi: 10.1371/journal.ppat.1010924. PMID: 36383559; PMCID: PMC9668162.

Structure of the TnsB transposase-DNA complex of type V-K CRISPR-associated transposon.  Tenjo-Castaño F, Sofos N, Lopez-Mendez B, Stutzke L, Fuglsang A, Stella S, Montoya G. Nat Commun 2022 13, 5792. doi.org/10.1038/s41467-022-33504-5

Transposons and CRISPR: Rewiring Gene Editing. Tenjo-Castaño F, Montoya G, Carabias A. Biochemistry. 2022 Sep 21. doi: 10.1021/acs.biochem.2c00379. (Review)

Structure of the RAF1-HSP90-CDC37 complex reveals the basis of RAF1 regulation. García-Alonso S, Mesa P, Ovejero LP, Aizpurua G, Lechuga CG, Zarzuela E, Santiveri CM, Sanclemente M, Muñoz J, Musteanu M, Campos-Olivas R, Martínez-Torrecuadrada J, Barbacid M, Montoya G. Molecular Cell. 2022 Sep 15;82(18):3438-3452.e8. doi: 10.1016/j.molcel.2022.08.012. Epub 2022 Sep 1.

A new class of biological ion-driven rotary molecular motors with 5:2 symmetry. M Rieu, R Krutyholowa, NMI Taylor, RM Berry. Frontiers Microbiol. 2022 doi.org/10.3389/fmicb.2022.948383, 13:948383. (Review)

Structures of Atm1 provide insight into [2Fe-2S] cluster export from mitochondria. Li P, Hendricks AL, Wang Y, Villones RLE, Lindkvist-Petersson K, Meloni G, Cowan JA, Wang K, Gourdon P. Nature Communications 2022 Jul 27;13(1):4339. doi: 10.1038/s41467-022-32006-8.

Structural basis of torque generation in the bi-directional bacterial flagellar motor. Hu H, Santiveri M, Wadhwa N, Berg HC, Erhardt M, Taylor NMI. Trends Biochem Sci. 2022 Feb;47(2):160-172. doi: 10.1016/j.tibs.2021.06.005. (Review)

Cryo-EM structure of native human thyroglobulin. Adaixo R, Steiner EM, Righetto RD, Schmidt A, Stahlberg H, Taylor NMI. (2022) Nature Communications Jan 10;13(1):61. doi: 10.1038/s41467-021-27693-8.

Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3.
Carabias A, Fuglsang A, Temperini P, Pape T, Sofos N, Stella S, Erlendsson S, Montoya G. Nat Commun. 2021 Jul 22;12(1):4476. doi: 10.1038/s41467-021-24707-3.

Structural basis of torque generation in the bi-directional bacterial flagellar motor.
Hu H, Santiveri M, Wadhwa N, Berg HC, Erhardt M, Taylor NMI. Trends Biochem Sci. 2021 Jul 19:S0968-0004(21)00139-0. doi: 10.1016/j.tibs.2021.06.005. Online

Structure and transport mechanism of P5B-ATPases.
Li P, Wang K, Salustros N, Grønberg C, Gourdon P.  Nat Commun. 2021 Jun 25;12(1):3973. doi: 10.1038/s41467-021-24148-y. PMID: 34172751; PMCID: PMC8233418

Structure and Function of Stator Units of the Bacterial Flagellar Motor.
Santiveri M, Roa-Eguiara A, Kühne C, Wadhwa N, Hu H, Berg HC, Erhardt M, Taylor NMI.
Cell. 2020 Oct 1;183(1):244-257.e16. doi: 10.1016/j.cell.2020.08.016. Epub 2020 Sep 14.
PMID: 32931735

Structures of the Cmr-β Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas.
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G.
Mol Cell. 2020 Sep 3;79(5):741-757.e7. doi: 10.1016/j.molcel.2020.07.008. Epub 2020 Jul 29.
PMID: 32730741

Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent.
Bloch M, Santiveri M, Taylor NMI.
Methods Mol Biol. 2020;2127:227-244. doi: 10.1007/978-1-0716-0373-4_16.
PMID: 32112326

Structural basis of CRISPR-Cas Type III prokaryotic defence systems.
Molina R, Sofos N, Montoya G.
Curr Opin Struct Biol. 2020 Jul 23;65:119-129. doi: 10.1016/j.sbi.2020.06.010. Online ahead of print.
PMID: 32712502 Review

CRISPR-Cas12a: Functional overview and applications.
Paul B, Montoya G.
Biomed J. 2020 Feb;43(1):8-17. doi: 10.1016/j.bj.2019.10.005. Epub 2020 Feb 5.
PMID: 32200959 Free PMC article. Review.

Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas.
Molina R, Stella S, Feng M, Sofos N, Jauniskis V, Pozdnyakova I, López-Méndez B, She Q, Montoya G.
Nat Commun. 2019 Sep 20;10(1):4302. doi: 10.1038/s41467-019-12244-z.
PMID: 31541109 Free PMC article

High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage.
Slaymaker IM, Mesa P, Kellner MJ, Kannan S, Brignole E, Koob J, Feliciano PR, Stella S, Abudayyeh OO, Gootenberg JS, Strecker J, Montoya G, Zhang F.
Cell Rep. 2019 Mar 26;26(13):3741-3751.e5. doi: 10.1016/j.celrep.2019.02.094.
PMID: 30917325 Free PMC article.

Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity.
Stella S, Mesa P, Thomsen J, Paul B, Alcón P, Jensen SB, Saligram B, Moses ME, Hatzakis NS, Montoya G.
Cell. 2018 Dec 13;175(7):1856-1871.e21. doi: 10.1016/j.cell.2018.10.045. Epub 2018 Nov 29.
PMID: 30503205

 

 

7Z12
7Z1H
7OP8
7OP5
7OP1
7OP3
7ODF 
6QZT 
6GTC 
6GTD  
6GTE  
6GTG, 
6GTF
6S6B 
6SIC 
6S8B 
6S91 
6S8E  
6SHB 
6SH8
6YKM
6YKP
6YKR 

 

EMD-14438
EMD-14446
EMD-13014
EMD-13013
EMD-13011
EMD-13012
EMD-12827
EMD-10828
EMD-10829
EMD-10830
EMD-10831
EMD-10832

EMD-4691
EMD-0061
EMD-0062
EMD-0063
EMD-0065
EMD-0064 
EMD-10102 
EMD-10209 
EMD 10117
EMD: 10126 
EMD: 10119 
EMD: 10197
EMD: 10196 

 

All publications that include data generated at the Cryo-EM facility must include the following statement: Data collection was performed at the Danish Cryo-EM Facility at CFIM, University of Copenhagen, supported by Novo Nordisk Foundation (Grant agreement NNF14CC0001)

 

 

 

Cryo-EM Application Portal Denmark

Guillermo Montoya, Tillmann Pape