Proteomics couples electrical remodelling to inflammation in a murine model of heart failure with sinus node dysfunction
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Proteomics couples electrical remodelling to inflammation in a murine model of heart failure with sinus node dysfunction. / Kahnert, Konstantin; Soattin, Luca; Mills, Robert W; Wilson, Claire; Maurya, Svetlana; Sorrentino, Andrea; Al-Othman, Sami; Tikhomirov, Roman; van de Vegte, Yordi J; Hansen, Finn B; Achter, Jonathan; Hu, Wei; Zi, Min; Smith, Matthew; van der Harst, Pim; Olesen, Morten S; Olsen, Kristine Boisen; Banner, Jytte; Jensen, Thomas H L; Zhang, Henggui; Boyett, Mark R; D'Souza, Alicia; Lundby, Alicia.
In: Cardiovascular Research, Vol. 120, No. 8, 2024, p. 927–942.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Proteomics couples electrical remodelling to inflammation in a murine model of heart failure with sinus node dysfunction
AU - Kahnert, Konstantin
AU - Soattin, Luca
AU - Mills, Robert W
AU - Wilson, Claire
AU - Maurya, Svetlana
AU - Sorrentino, Andrea
AU - Al-Othman, Sami
AU - Tikhomirov, Roman
AU - van de Vegte, Yordi J
AU - Hansen, Finn B
AU - Achter, Jonathan
AU - Hu, Wei
AU - Zi, Min
AU - Smith, Matthew
AU - van der Harst, Pim
AU - Olesen, Morten S
AU - Olsen, Kristine Boisen
AU - Banner, Jytte
AU - Jensen, Thomas H L
AU - Zhang, Henggui
AU - Boyett, Mark R
AU - D'Souza, Alicia
AU - Lundby, Alicia
N1 - © The Author(s) 2024. Published by Oxford University Press on behalf of the European Society of Cardiology.
PY - 2024
Y1 - 2024
N2 - AIMS: In patients with heart failure (HF), concomitant sinus node dysfunction (SND) is an important predictor of mortality, yet its molecular underpinnings are poorly understood. Using proteomics, this study aimed to dissect the protein and phosphorylation remodelling within the sinus node in an animal model of HF with concurrent SND.METHODS AND RESULTS: We acquired deep sinus node proteomes and phosphoproteomes in mice with heart failure and SND and report extensive remodelling. Intersecting the measured (phospho)proteome changes with human genomics pharmacovigilance data, highlighted downregulated proteins involved in electrical activity such as the pacemaker ion channel, Hcn4. We confirmed the importance of ion channel downregulation for sinus node physiology using computer modelling. Guided by the proteomics data, we hypothesized that an inflammatory response may drive the electrophysiological remodeling underlying SND in heart failure. In support of this, experimentally induced inflammation downregulated Hcn4 and slowed pacemaking in the isolated sinus node. From the proteomics data we identified proinflammatory cytokine-like protein galectin-3 as a potential target to mitigate the effect. Indeed, in vivo suppression of galectin-3 in the animal model of heart failure prevented SND.CONCLUSION: Collectively, we outline the protein and phosphorylation remodeling of SND in heart failure, we highlight a role for inflammation in electrophysiological remodelling of the sinus node, and we present galectin-3 signalling as a target to ameliorate SND in heart failure.
AB - AIMS: In patients with heart failure (HF), concomitant sinus node dysfunction (SND) is an important predictor of mortality, yet its molecular underpinnings are poorly understood. Using proteomics, this study aimed to dissect the protein and phosphorylation remodelling within the sinus node in an animal model of HF with concurrent SND.METHODS AND RESULTS: We acquired deep sinus node proteomes and phosphoproteomes in mice with heart failure and SND and report extensive remodelling. Intersecting the measured (phospho)proteome changes with human genomics pharmacovigilance data, highlighted downregulated proteins involved in electrical activity such as the pacemaker ion channel, Hcn4. We confirmed the importance of ion channel downregulation for sinus node physiology using computer modelling. Guided by the proteomics data, we hypothesized that an inflammatory response may drive the electrophysiological remodeling underlying SND in heart failure. In support of this, experimentally induced inflammation downregulated Hcn4 and slowed pacemaking in the isolated sinus node. From the proteomics data we identified proinflammatory cytokine-like protein galectin-3 as a potential target to mitigate the effect. Indeed, in vivo suppression of galectin-3 in the animal model of heart failure prevented SND.CONCLUSION: Collectively, we outline the protein and phosphorylation remodeling of SND in heart failure, we highlight a role for inflammation in electrophysiological remodelling of the sinus node, and we present galectin-3 signalling as a target to ameliorate SND in heart failure.
U2 - 10.1093/cvr/cvae054
DO - 10.1093/cvr/cvae054
M3 - Journal article
C2 - 38661182
VL - 120
SP - 927
EP - 942
JO - Cardiovascular Research
JF - Cardiovascular Research
SN - 0008-6363
IS - 8
ER -
ID: 393772433